Technology

3D laser bioprinting is a new approach based principally on Laser Induced Forward Transfer (LIFT), addressing the great demand for 3D-improve tissue models on lab automation, especially for pharmaceutical and cosmetics industry. The technical is a contact free process for precise cell printing, allowing thus external foreign pollutants and fully organic printing approach, with or without additional supporting and absorbing additives. For conventional LIFT technology, the transferred material is supported by a sacrificial thin layer, both metallic or polymer, which facilitates the ejected material after evaporation due to thermal-mechanical effect, either by direct procedure generated into the culture medium or an biogel. In consequence, laser assisted bioprinting (LAB) tecnology will lead to more solid and better tissue models, which would replace animal experiments or simplify certains experimental phase.

LAB appears as the best solution in the bioprinting domain, due to considerable advantages as resolution (single cell level), precision (micron scale) and post printing cell viability (> 90 %), improving the reproducibility to adapt 3D cellular structure to native tissue by depositing cells and extracellular matrix or in general tissue components layer by layer. Highlight, LAB technologies should be used not only for tissue printing, could also be used for cell sorting and picking in the immunological and genetics sector.

From left to right: Cell recognition; Optics and positional identify process; Cell transfer process analysis of jetting dynamics (LIFT); Cellular matrix deposited on hydrogel substrate:

 

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